The King of Limbs band, who unveiled new track 'Staircase' earlier this week, have been confirmed to appear at the music event following Emily Eavis's teaser that there will be a surprise performance from an act good enough to headline.
Radiohead will play the Park Stage at 8pm this evening, according to the band's spokesperson, reports NME.
Radiohead have played Glastonbury four times before. They made their first appearance in 1994, leading on to headline slots at the event in both 1997 and 2003.
Last year, band members Thom Yorke and Jonny Greenwood played a short set on the Park Stage, causing a mass surge of festival-goers to the area.
Headline acts for this weekend's Glastonbury festival include U2, Coldplay and Beyoncé.
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Watch Radiohead perform 'Paranoid Android' at Glastonbury in 2003 below: