As seen in the video below, the producer and close friend of Winehouse used the show as an opportunity to pay tribute to the 'Back To Black' singer, who tragically passed away aged 27 in her Camden home on Saturday.
It was the first gig Ronson had performed since Winehouse's death. He kicked off the performance with a rendition of 'Valerie' and invited members of the star's backing band on stage to play the track with him, reports BBC Newsbeat.
The Zutons singer Dave McCabe, who originally recorded the track, took lead vocals.
"She made brilliant music, more than I'll make in my entire life," Ronson told the audience at the Greenwich Summer Series festival.
During a short DJ set in the middle of the concert, the Brit Award-winning producer played Winehouse's iconic track 'Rehab'.
He was then joined on stage by The Rumble Strips' lead singer Charlie Waller for a full band cover of 'Back To Black'.
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"It's really lovely getting to play some music here for you tonight," Ronson said from the stage. "That's what makes everything better.
"I went to her service yesterday and there was a rabbi that spoke and he said that somebody's life is measured in deeds and not years and that's the best thing I heard yesterday."
He added: "The genius in that woman and what she shared with us is pretty special. I'm not going to get all morbid on you. It's just nice to be playing music to people who like good music. She is my sister, wherever she is."
Following Amy Winehouse's death on Saturday, fans have been remembering the star through her music.
Winehouse tallied up seven re-entries in the UK midweek chart yesterday, with her Grammy Award-winning album Back To Black currently at number two in the UK chart and number nine on the US Billboard 200.
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Watch Mark Ronson perform 'Valerie' in tribute to Amy Winehouse last night below: