The Take That singer has called upon N-Dubz, Chipmunk, Rizzle Kicks, Ed Sheeran, Wretch 32, Tinchy Stryder, Ms. Dynamite, Mz Bratt and Dot Rotton for the track, while Labrinth has been given the role of producer.
The song will be a reworked cover of Massive Attack's 'Teardrop', with the proceeds to be donated to this year's Children In Need.
Speaking to The Sun, Barlow explained: "I think this is a brilliant project. Children In Need has a history of doing upbeat singles with comedians but it's a serious charity doing a lot of hard work for serious causes.
"It's time to do something with artists who young people actually listen to. I've done these things before, I'm used to doing group situations, I like it - it's a challenge. It hasn't been easy. But bless him, Labrinth and his manager have been the key to the whole thing.
"I met up with them and they started doing the whole lot, talking to friends etc, and all of a sudden the word started to get out. Hopefully we should be nailed on for a number one - and raise loads of money in the process."
The artists have yet to come up with a name for their newly-formed musical collective, but confirmed that the single will be released on November 13.
> Gary Barlow: 'I've been selfish with Take That'
Watch the music video for Massive Attack's 'Teardrop' below: