The track, which has managed a total of nine weeks on the chart, holds off Maroon 5 and Christina Aguilera's 'Moves Like Jagger', which remains at two, while Jason Derulo's 'It Girl' completes the top three.
Meanwhile, Cobra Starship and Sabi's 'You Make Me Feel' jumps from 15 to six, and David Guetta and Usher's 'Without You' vaults from 25 to ten.
Outside the Top 10, Jessie J debuts at 12 with 'Domino', Adele's 'Set Fire to the Rain' leaps from 40 to 26, and Calvin Harris advances from 36 to 28 with 'Feel So Close'.
Further down the chart, Nicki Minaj and Rihanna land at 38 with 'Fly' and Beyoncé's 'Love On Top' starts at 40.
The Top 10 singles in full (click where possible for our reviews):
1. (1) Gotye ft. Kimbra: 'Somebody That I Used To Know'
2. (2) Maroon 5 ft. Christina Aguilera: 'Moves Like Jagger'
3. (4) Jason Derulo: 'It Girl'
4. (3) Adele: 'Someone Like You'
5. (5) David Guetta ft. Sia: 'Titanium'
6. (15) Cobra Starship ft. Sabi: 'You Make Me Feel'
7. (8) Rihanna: 'Cheers (Drink To That)'
8. (7) Calvin Harris: 'Bounce'
9. (13) Adele: 'Rolling in the Deep'
10. (25) David Guetta ft. Usher: 'Without You'
Watch Gotye's 'Somebody That I Used To Know' video below: