The band will broadcast Pearl Jam Twenty across 67 cinemas tonight, in what will be a one-off event.
The documentary showcases a definitive portrait of the group's 20-year career and has been directed by Academy Award-winning filmmaker and music journalist Cameron Crowe.
"When I set out to make this film, my mission was to assemble the best of the best from Pearl Jam's past and present and give audiences a visceral feeling of what it is to love music and to feel it deeply - to be inside the journey of a band that has carved their own path," said Crowe.
"There is only one band of their generation for which a film like this could even be made, and I'm honoured to be the one given the opportunity to make it."
Pearl Jam Twenty will give fans an intimate glimpse into Pearl Jam's journey, which has been edited from over 1,200 hours of rare footage.
The film's soundtrack is available to buy now.
Pearl Jam recently announced plans to continue celebrating their 20th anniversary by performing at a special charity concert next month.
Watch Pearl Jam perform 'Better Man' live in New York City below: