The group will appear at 'Michael Forever', which takes place at Cardiff's Millennium Stadium on October 8. Christina Aguilera, Ne-Yo, Cee Lo Green, Alexandra Burke and Leona Lewis are among the other artists who have confirmed to perform so far.
will.i.am explained that he and his bandmates have been massive fans of the late singer, and have always paid tribute to Jackson at each of their live shows since his death in 2009.
"This show is going to be an incredibly personal and moving evening for me," the frontman said.
"Remember that Michael's music, his great, great songs, have been an inspiration to my whole life and certainly formed the soundtrack to my childhood. When I was a little kid of 6 or 7 growing up in Los Angeles, there was Michael recording Thriller, and all his early hits, just a few miles away across town - obviously I didn't know him at that time, but I loved him as a fan.
"Then one day about four years ago, with The Black Eyed Peas well established, I got a call from him I will never forget. I then had the great privilege of working with him and getting to know him and his beautiful family.
"That's why The Black Eyed Peas wouldn't miss being at the Millennium Stadium for this gig on 8 October; we all owe Michael such a lot. It seems plain to me that the real star of this tribute to Michael is going to be the music itself. And that's the only way it should be."
Of their involvement, brother Marlon Jackson added: "We're all really delighted and touched that the Black Eyed Peas are able to come and take part. My brother rated will.i.am and the original things he does in music very highly. I guess you could say it was a case of mutual respect."
Jamie Foxx has been confirmed as the host of the event next month.
The full list of artists performing at 'Michael Forever' so far are: Black Eyed Peas, Jennifer Hudson, Christina Aguilera, Cee Lo Green, Gladys Knight, Smokey Robinson, Leona Lewis, The Jackson Brothers, Alexandra Burke, JLS, Diversity, Ne-Yo, Pixie Lott, Alien Ant Farm, Jamie Foxx, 3T and Craig David.
Watch Black Eyed Peas and Michael Jackson in the studio together from 2006 below: