The 'Marry the Night' singer and Aldean will take to the stage live on November 30 for the televised event.
The Grammys announced the news via their Twitter page on Monday night, with details of more artists expected to be released over the coming days.
The 54th Grammy Awards will be held on February 12, 2012 at the Staples Centre in Los Angeles.
Earlier this year, the Recording Academy announced several changes to the Grammys, including the reduction of categories from 109 to 78 for next year's event.
Lady GaGa recently serenaded former US President Bill Clinton for his 65th birthday.
The Grammy Nominations Concert Live! - Countdown To Music's Biggest Night will air on CBS.
> Lady GaGa shoots new 'Marry the Night' video in New York
Watch Lady GaGa's performance of 'Born this Way' at the 53rd Grammy Awards below: