The Stone Roses pair, who announced the full reunion of the 'Fools Gold' band in October, shared the stage at Manchester's The Ritz as part of the Justice Tonight campaign gig on Friday night.
Brown and Squire played 'Bankrobber' with Mick Jones, before performing Stone Roses classic 'Elizabeth My Dear', reports Louder Than War.
The Justice Tonight series of gigs is being held in aid of the Hillsborough Justice Campaign, which fights on behalf of those killed in the 1989 football ground disaster.
Brown and Squire famously fell out and did not speak to each other for years after the band originally parted ways.
The Stone Roses have announced a string of comeback shows across the UK and Ireland, which sold out in a matter of hours. They have also been confirmed for a number of festival appearances, including T In The Park , Spain's Benicassim festival and Denmark's NorthSide festival.
> David Beckham 'helped Stones Roses reunion'
> Shane Meadows: 'Stone Roses reunion film will make up for missed gig'
Watch a video of Ian Brown and John Squire performing together below:
Move through our images from the Stone Roses' career below: