The Grammy Award-winning entertainer was announced tonight (December 4) as the main performer at the annual sports event by NBC.
It was first rumoured that Madonna would be performing at the Super Bowl in October.
According to the official press release, her performance will be 'imagined' by Cirque du Soleil, choreographer Jamie King and multimedia artists Moment Factory.
The Super Bowl halftime show is the most-watched musical event of the year in the US, with over 162 million viewers tuning in to watch last year's performance by the Black Eyed Peas.
Other past acts at the event include The Who, Bruce Springsteen, Michael Jackson, Tom Petty, Paul McCartney and U2.
Madonna is currently working on her new album for release next year, while her latest film W.E will open in the US on February 3.
Super Bowl XLVI takes place on February 5, 2012 at the Lucas Oil Stadium, Indianapolis.
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Watch the Black Eyed Peas' Super Bowl performance last year below: