The twins unveiled footage of themselves ripping off their T-shirts and stripping naked to thank fans for getting them to 500,000 followers on the micro-blogging site.
John and Edward dance seductively to LMFAO's hit 'Sexy And I Know It' and named their dance routine to the track as 'Jexy And I Know It'.
The clip was uploaded to YouTube over the weekend and has since been flagged as inappropriate for under-18-year-olds by the video-sharing site.
Jedward recently confirmed plans to represent Ireland in the Eurovision Song Contest for a second time in 2012, telling Digital Spy that it's going to be "huge".
> Jedward party with Tara Reid in 'Wow Oh Wow' video - watch
Watch Jedward strip down in their 'Jexy And I Know It' video below: