The Blur star will perform his Doctor Dee opera at the music festival, which will take place in Rockley, Wiltshire on April 14.
It will be a chance for fans to see the show ahead of the opera's run at London's Coliseum from June 25 later this year.
Dry the River, Raghu Dixit and Rae Morris have also been confirmed to play this spring's Honeyfest, which will be supported by the BBC in association with its BBC Introducing festival stage.
Tickets for the music event are on sale now and can be purchased from the festival's official website.
> Damon Albarn 'Doctor Dee' opera about "being English"
Watch Damon Albarn perform 'Apple Carts' from Doctor Dee below: