'Love Is Not Enough' is released on March 19, backed by remixes including the Above & Beyond Club Mix and a mix from MRZO (aka Mat Zo).
The Vicky Simmons-directed clip features Liam Boyle (Awaydays) and Emily Beecham (28 Weeks Later).
"The video for 'Love Is Not Enough' follows the thoughts and emotions of two lost lovers in the final moments of their relationship - just as they realise that the relationship really isn't going to work," Simmons said.
"The whole video is filmed and edited in reverse, reflecting upon the cyclical nature of relationships. The backwards footage highlights the ambiguity and uncertainty of whether they are emotionally leaving or returning."
The single is the latest cut to be released from the dance trio's 2011 album Group Therapy.
Above & Beyond tour the UK this spring, with support from Australian DJ Jaytech.
After their show on Saturday, April 7 sold out, the group added a second London date on Sunday, April 8.
Above & Beyond came fifth in last year's Top 100 DJs Poll, behind David Guetta, Armin Van Buuren, Tiesto and Deadmau5.