The lineup for the August Bank Holiday weekender will be announced tonight, but the recently-reformed group tweeted this afternoon that they would play.
"Freight train coming !!! ... At The Drive In will be performing at Reading / Leeds Festivals August 25-26," the band said.
At The Drive-In last played the Reading and Leeds Festivals in 2000, playing fifth from the top of the bill on the Carling Premier Stage.
The group confirmed their reunion in January after an 11-year break.
At The Drive-In split in 2001 and members from the group parted ways to form Sparta and The Mars Volta.
The group have already been confirmed for this year's Coachella festival in California.
At The Drive-In guitarist Omar Rodriguez-Lopez last month said that the reunion was "more of a nostalgia thing" and ruled out the possibility of a new album.
Watch the At The Drive-In 'One Armed Scissor' music video below: