The American Idol judge will perform as part of the Pop Music Festival in Sao Paolo on June 23.
Lopez will also stage a separate gig in Rio de Janeiro on June 27.
The announcement of Lopez's first concert in the region coincided with a tour of the country this week to promote her Spanish-language reality competition Q'Viva.
The 'On The Floor' singer recently unveiled her new single 'Dance Again', which is her latest collaboration with rapper Pitbull.
Lopez's most recent album Love? reached the top ten on album charts across the globe in 2011.
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Watch Jennifer Lopez's 'On The Floor' music video below: