The late rapper joined Dre and Snoop Dogg on stage to perform 'Hail Mary' through a hologram display on Sunday, April 15.
A rep for Afeni told TMZ that she agreed to the hologram after Dre asked for her approval.
The rep said that Afeni, who didn't attend the concert, was "thrilled" with the performance.
Dre showed his gratitude by donating to The Tupac Amaru Shakur Foundation.
Several guests also made appearances during Dre's concert, including Eminem, 50 Cent, Warren G, Kendrick Lamar and Wiz Khalifa.
Watch the hologram performance below: