The track will serve as the lead single for the electronic band's upcoming fifth studio album In Our Heads.
'Night and Day' received its first official play on Zane Lowe's BBC Radio 1 show earlier this evening (April 17), and will be released as a digital download and a 12" vinyl on June 4.
The digital download of the single will contain a 2 Bears remix featuring Trim, along with a B-side titled 'Jelly Babies'.
The band describe the track as "weird-ass disco", adding: "The song is driven by a menacing yet supremely danceable bassline, while Alexis's digitally-messed up vocals float beautifully above it all like the ghost of Marvin Gaye."
In Our Heads is released on June 12 in the US and June 11 in the UK via Domino Records.
Watch the video for Hot Chip's 'I Feel Better' below: