Little Boots will support the band's two London shows on May 16 and May 17 this year.
The singer, whose real name is Victoria Hesketh, tweeted: "Very excited to announce we will be supporting @scissorsisters at their shepherds bush shows next month! Fun times x"
Hesketh recently debuted new track 'Every Night I Say A Prayer', which was released in aid of this year's Record Store Day.
Last month, the star put out her third mixtape for free online, which included re-workings of Kylie Minogue's 'Slow' and Azari & III's 'Reckless With Your Love'.
Listen to 'Every Night I Say A Prayer' below: