The entire performance, recorded at DC's Washington Coliseum on February 11, 1964, will be screened as part of 92-minute documentary The Beatles: The Lost Concert.
The film charts the birth and impact of Beatlemania in America, and feature interviews with more than 20 Beatles associates, journalists, disc jockeys, concert attendees, historians and musicians, the latter including Chuck Berry and Mark Ronson.
The concert footage has been described as one of music's "lost treasures", having remained unseen in its entirety by audiences for over 47 years.
The original master tapes have been restored and remastered, and the recording represents the only complete Beatles concert available to fans.
Aerosmith star and American Idol judge Steven Tyler commented on the gig: "This blows away every performance I've ever seen, including Elvis!"
The documentary will receive a world premiere at New York's Ziegfield Theatre on May 6, and will subsequently be shown in cinemas across the US on May 17 and May 22.
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Watch a trailer for 2011 documentary George Harrison: Living in the Material World below: