The rapper - real name Donald Glover - will play a set at XOYO on July 4 in support of his debut album Camp, which was released last November.
The one-off headline set in London will precede his performances at Hyde Park's Wireless festival and Scotland's T in the Park on July 6.
Camp reached number 11 on the US Billboard 200 last year and spawned the singles 'Bonfire' and 'Heartbeat'.
Tickets for Childish Gambino's XOYO show will cost £15 and go on sale from 9am on June 22.
The star's new single 'Fire Fly' will follow on July 9.
Watch the music video for Childish Gambino's 'Heartbeat' below: