The rapper's latest track 'Whistle' has topped the chart, pushing Cheryl Cole's 'Call My Name' down to number two.
Maroon 5 are at three with 'Payphone', Loreen remains at four with 'Euphoria' and Coldplay and Rihanna are at five with 'Princess Of China'.
Katy Perry's 'Wide Awake' advances to 12, Paloma Faith's 'Picking Up The Pieces' vaults from 77 to 13 and Ireland's Euro 2012 anthem 'Rocky Road To Poland' falls to 16.
Stooshe land at number 19 with 'Black Heart', Usher is up to 27 with 'Scream' and Jedward's 'Young Love' enters at number 31.
This week's Irish Top 10 is as follows:
1. (3) Flo Rida: 'Whistle'
2. (1) Cheryl: 'Call My Name'
3. (5) Maroon 5 featuring Wiz Khalifa: 'Payphone'
4. (4) Loreen: 'Euphoria'
5. (7) Coldplay and Rihanna: 'Princess Of China'
6. (6) fun. featuring Janelle Monaé: 'We Are Young'
7. (8) Marina and the Diamonds: 'Primadonna'
8. (10) Train: 'Drive By'
9. (11) Nicki Minaj: 'Starships'
10. (12) Rihanna: 'Where Have You Been'
Watch the music video for Flo Rida's 'Whistle' below: