The singer is searching for 15 men to perform with her in front of 30,000 people at Pete Waterman's Hit Factory Live concert in Hyde Park next month.
The star will hold auditions at Madame JoJo's in London on Monday, July 2 from 1pm. The winners will be coached by Sinitta herself ahead of the show on July 11.
Other performers at the one-off event, which celebrates 25 years of Waterman's PWL record label, include Steps, Kylie Minogue, Bananarama, Jason Donovan, Sonia, 2 Unlimited and Dead or Alive.
Waterman recently admitted that he initially did not want to sign Donovan to his record label.
Watch Sinitta's 'So Macho' music video below: