Frontman Chris Martin sang a line from the boyband's global hit 'What Makes You Beautiful' during the band's rendition of 'Yellow' in Tampa, Florida, USA.
The star referenced One Direction as an acknowledgement of Harry Styles being present at the gig.
The shout-out comes under two years since One Direction performed Coldplay's hit 'Viva la Vida' during their first live show performance on The X Factor in 2010.
Both Coldplay and One Direction are currently on headline tours of North America in support of their respective records Mylo Xyloto and Up All Night.
Meanwhile, Martin recently described Coldplay as the Manchester United of music, saying that the group will always encounter negative opinion, despite being one of the most successful music acts in the world.
Watch Chris Martin sing a line from One Direction's 'What Makes You Beautiful' below: