The Australian performers will perform 'Especially For You' at this week's PWL Hit Factory Live concert in London's Hyde Park.
The pictures sees Minogue and Donovan with their backing band in a rehearsal space in London.
Minogue tweeted the image with the caption: "Rehearsals with gorgeous @JDonOfficial for @PWLHitFactory."
The 'Timebomb' performer revealed that she would be duetting with her former on and off-screen love interest last month.
''I'm going to sing 'Especially for You' with Jason Donovan," Minogue told Glamour.
"I don't think we'll even need to sing it. I'm sure the audience all went through the Neighbours wedding. It's going to bring the house down! Even though it's outdoors.''
PWL Hit Factory Live takes place on Wednesday (July 11), and will feature performances from Steps, Dead Or Alive, Sinitta, 2 Unlimited, Bananarama and Rick Astley.
Watch the music video for Kylie Minogue and Jason Donovan's 'Especially For You' below: