The Florence Welch-fronted act have topped the UK midweek chart update with a Calvin Harris remix of their single 'Spectrum'.
Maroon 5 slip to two with 'Payphone', while will.i.am is at three with 'This Is Love'.
Chris Brown slides a couple of places to four with 'Don't Wake Me Up' and Stooshe remain firm at five with 'Black Heart'.
Nicki Minaj is at 10 with 'Pound The Alarm' and Adam Lambert is just outside the Top 10 at 13 with 'Never Close Our Eyes'.
David Guetta vaults 200 places up to 23 with 'I Can Only Imagine', which features Chris Brown and Lil Wayne, while The Vaccines are on course to claim their highest charting single to date with 'No Hope' at 30.
Over on the album chart, Newton Faulkner lands at number one with new record Write It On Your Skin.
Brown's Fortune slips to five, Adam Lambert's Trespassing is at seven and Frank Ocean's channel ORANGE is heading for a Top 10 debut.
Watch the music video for Florence + the Machine's 'Spectrum' below: