The late singer's cover of The Zutons' track 'Valerie' has sold 767,000 copies to date, a combined total of her solo version and collaboration with Mark Ronson.
Winehouse - who passed away a year ago today (July 23) - has sold 1.7 million records since her death, including 1.2 million copies of her three records.
Official Charts Company managing director Martin Talbot said: "Many of us will remember where we were when we heard the terrible news about Amy a year ago.
"And the fact that music fans have, since then, bought her music in such large numbers demonstrates just how highly she was regarded by the great British public."
The star's 2006 hit 'Rehab' finished second place on the list, followed by 'Back To Black' and 'Tears Dry On Their Own', while her duet with Tony Bennett 'Body & Soul' completes the top ten.
Meanwhile, Winehouse's Back To Black LP remains her biggest-selling album with sales of 3.5 million, while her posthumous album Lioness: Hidden Treasures has shifted 750,000 copies in under a year.
The top ten Amy Winehouse tracks are as follows:
1. 'Valerie' (Amy Winehouse/Amy Winehouse ft. Mark Ronson combined)
3. 'Back To Black'
4. 'Tears Dry On Their Own'
5. 'You Know I'm No Good'
6. 'Love is a Losing Game'
7. 'Our Day Will Come'
8. 'Will You Still Love Me Tomorrow'
9. 'Monkey Man'
10. 'Body & Soul' (ft. Tony Bennett)
Click through our gallery below of Amy Winehouse: One year after her death below: