The US five-piece will play Camden's Roundhouse venue on September 19.
Matchbox Twenty told Metro that they will do their "favourite thing" for the UK audience, adding that gigging is "always a win" and that London is one of their "favourite cities".
Other artists confirmed for this year's month-long series of gigs include Pink, deadmau5 and David Guetta.
Tickets for the show are free for competition winners, and can be applied for on the festival website.
Matchbox Twenty release their new studio album North this September. It was preceded by the single 'She's So Mean' last month.
Watch the Matchbox Twenty 'She's So Mean' lyric video below: