The band will perform at London's Roundhouse on September 11 prior to the release of their new album Battle Born on September 17.
The show is in addition to another free gig in support of the record, which will take place at London's HMV Forum on September 10.
Usher, Pink, One Direction, Emeli Sandé, Plan B, JLS and Jessie J are among the other artists scheduled to perform at the iTunes Festival.
Ellie Goulding, Mumford & Sons, Example, Calvin Harris and Olly Murs will also appear.
Fans can apply for tickets to any of the shows on the iTunes Festival website.
Watch The Killers' video for 'Runaways' below: