The band will play London's Wembley Arena on December 8 in support of their 2011 album Sinners Never Sleep.
You Me At Six will also officially release 'Reckless' as their new single from the album on October 22.
To celebrate their biggest headline show to date, the group will team up with Abbey Road Live Here Now to record and film the concert.
The 'Final Night of Sin' show will then be made available as a unique limited edition 3-disc CD/DVD, 2-disc audio CD or an MP3 download.
Tickets for the show go on sale from 9am on August 29.
Meanwhile, the band will play the Main Stage at this weekend's Reading and Leeds Festival.
Listen to You Me At Six's new single 'Reckless' below: