The music event, which will be held in Philadelphia, PA over Labour Day weekend, is said to include a "top secret" appearance by the 'I Was Here' singer on one of the two days.
"Beyonce will hit the stage in a surprise performance at her hubby's festival next weekend in [Pennsylvania]," an insider told The Huffington Post.
"The performance is top secret but it will be filmed for Jay's documentary about the event. It's going to be epic."
Skrillex, Drake, Afrojack, Chris Cornell, Run-DMC and Jill Scott are also scheduled to perform at the Made in America festival, which will kick off on September 1.
It was recently announced that filmmakers Ron Howard and Brian Grazer will shoot the festival, which will later be released as a movie.
Watch Beyoncé's video for 'I Was Here' below: