The two musicians are currently travelling together on a North American tour, which was to stop in Orlando, Florida on Sunday (September 2).
The planned concert at the Amway Centre has been scrapped due to what representatives of the venue have called "logistical issues."
"The tour will now conclude with two final shows at AmericanAirlines Arena in Miami, Florida on August 31 and September 1," a venue statement explained. "Ticket holders for the September 2nd Orlando date will be refunded at original point of purchase."
This is the second concert cancelation on the pair's joint tour. A show in Anaheim, California was also axed earlier in the month.
Lopez recently confirmed plans for a 3D concert film.
Watch the music video to 'Dance Again' by Jennifer Lopez below: