The DJ has reworked the reggae star's 1977 track 'Jammin', which originally appeared on his ninth studio album Exodus.
The track will be available to download from September 3 and can be heard in full for the first time exclusively on Digital Spy below.
'Jammin' is the first official Marley remix since the Funkstar Deluxe remix of 'Sun Is Shining' back in 1999.
Bob Marley released 13 studio albums during his lifetime and sales of his music has surpassed 21 million records worldwide since records began in 1991.
Listen to Benny Benassi's remix of Bob Marley's 'Jammin' below: