Taking place between September 7 and 9, it is to be the first time the music event has been broadcast live on the video-sharing site.
Fans from across the globe will be able to tune into the action via the festival's official YouTube channel.
Florence + the Machine, New Order, The xx, Two Door Cinema Club, Emeli Sandé, Orbital and Azealia Banks are among the acts viewers will be able to watch.
"I've been watching festivals such as Coachella and Tomorrowland on YouTube and loving the futuristic new angle on showing festivals in a different light via a medium that nearly everyone in the music and festival worlds is tuned into," said Bestival promoter Rob da Bank.
"We're really pleased that Bestival is the first UK festival to go down this route."
Once Bestival comes to a close, individual songs from acts will be available on-demand for the next 90 days on the Bestival YouTube channel.
Watch a trailer for Bestival's live YouTube coverage below: