Three people were killed in the incident as the coach was returning to Aintree from the Isle of Wight festival.
The DJ stated his shock upon hearing of the tragedy, and sent out his wishes from the Bestival team to the victims' friends and family.
"I just wanted to personally send my condolences and heartfelt sorrow to the families and friends of everyone affected by the coach crash after Bestival," he said via Facebook.
"Myself, Josie, John, Ziggy, Ben and the whole Bestival family and production team are devastated that this has happened at the end of what was hopefully a joyous weekend of music and fun for most and leaves a shadow over all of us.
"Bestival is a festival that thrives on positive music and good vibes so this has been a terrible shock. We're in constant touch with Surrey Police and are assisting in any way we can."
Two of the three victims have been named as 63-year-old coach driver Colin Daulby and musician Michael Molloy, 18, both of Merseyside. The third victim is yet to be identified.
It is believed that Daulby may have suffered a heart attack while driving the coach, reports the Daily Telegraph.
A casualty number has been set up by police, with any people concerned about passengers on the bus instructed to call 08000 560 154 for information.