The gig at the 20,000 capacity venue is the band's biggest ever UK headline show.
Tickets are available via a presale this Wednesday, September 19 and go on general sale on Friday, September 21 at 9am and are priced at £26.
The band take their second album The Vaccines Come Of Age on the road this November.
The Vaccines release new single 'I Always Knew' via Columbia Records on November 11 on digital download and 7" vinyl.
Following the theme of each band member contributing a B-side, the single features 'Make You Mine', written and sung by drummer Pete Robertson.
The bonus track was produced by Matt Boynton at Vacation Island Studios in Brooklyn.