The group recently performed an end-of-tour gig at the Global Citizen Festival in Central Park on Saturday (September 29).
After the show, Grohl revealed to The Huffington Post that they plan on going on a long hiatus.
He said: "Without making a big deal out of it, we don't have any shows after this. This is it, man.
"This is the show where we come out and we play as many songs as we can in a short period of time, because, honestly, I don't know when we're gonna do it again and this is the perfect place to do it, right now, tonight."
Grohl recently downplayed a comment made after the band performed at Reading Festival, where he said it would be their "last show" for some time.
There is no word yet on exactly how long the band's hiatus is expected to be.