The singer was performing 'Edge of Glory' in Barcelona as part of her 'Born This Way Ball' world tour when the incident occurred.
Fan footage sees the star vomit at the back of the set, while a dancer notices and performs in front of her to obstruct the audience's view.
After throwing up three times in a number of seconds, GaGa struts to the front of the stage to continue with the show.
The incident happened just days after a video of Justin Bieber vomiting in concert appeared on the internet.
Meanwhile, Lady GaGa became the first person on Twitter to accumulate 30 million followers last week.
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