The 'Fire with Fire' group confirmed at their gig at Camden's Roundhouse in London last night (October 24) that they currently have no plans to work together again.
The band played a second of two nights at the venue, putting on an extended gig to thank their fans, before making the announcement towards the end of the show.
Jake Shears told the crowd: "This will be the last time we will play in London for quite some time."
Ana Matronic thanked their fans, stating that the UK audience were "the ones who got us first - and always get us best".
The New York group played a selection of their biggest hits at the gig, including 'Laura', 'Mary' and 'Kiss You Off', before closing the show with 'Filthy/Gorgeous'.
They also performed the singles 'Fire with Fire', 'Invisible Light', 'Only the Horses', 'Shady Love', 'I Don't Feel Like Dancing', 'Take Your Mama' and 'Comfortably Numb'.
After the performance, the band confirmed to Gigwise that they currently have no plans to return to the studio, with guitarist Del Marquis revealing that he is currently working on solo material.
However, Ana Matronic also reassured fans that this was not necessarily the end of Scissor Sisters altogether, saying: "Don't you worry - this isn't the last you've heard of us."
Watch the video for 'Let's Have a Kiki' by Scissor Sisters below: