The electronic music magazine has shortlisted nearly 300 tracks across genres, from techno to dubstep to drum 'n' bass, while fans can also add their own choice if it's not listed.
Mixmag editor Nick Decosemo said: "Of all the polls we've ever run at Mixmag, this one has caused the most office arguments!
"Picking one greatest dance track of all time seems almost like an impossible task to many."
He added: "So to help people along, we've created a list of 300 timeless records that we think could be in contention to win.
"You can listen to all of these tunes on our bespoke Facebook App and then make your choice. Just be careful, once you start browsing you may find that you've lost a day!"
Voters can also buy songs on the shortlist with a 20% discount via Beatport using a code on the poll's homepage.
The poll will run until December 21, 2012 and the winner will be revealed in the February
issue of Mixmag, on sale January 17, 2013.