The 'U Can't Touch This' star joined Korean singer and rapper Psy for a rendition of the latter's international hit 'Gangnam Style' during Sunday night's ceremony (November 18).
Psy took to the stage at the end of the show sporting a pair of black, baggy trousers similar to the 'Hammer pants' popularised by MC Hammer at the height of his fame.
The pair combined moves from 'Gangnam Style' and '2 Legit 2 Quit' when MC Hammer appeared on stage halfway through the song.
[Psy, wearing 'Hammer pants', poses for a picture with MC Hammer in the American Music Awards press room]
Justin Bieber was the night's major winner, taking home three awards including the 'Artist of the Year' trophy.
Nicki Minaj, Carrie Underwood, David Guetta and Carly Rae Jepsen were also among this year's American Music Awards winners.
Watch MC Hammer's music video for 'U Can't Touch This' below: