The rapper will top the bill at the Glasgow Summer Sessions at Bellahouston Park, which will take place over ten days from August 20.
"His performance at T in the Park and his previous stadium show at Hampden were spectacular, so this gig will be one of the events of the year," said Geoff Ellis, chief executive of organisers DF Concerts.
"As it's one of the most important music destinations in the world, we're also excited about what else we have to announce for Summer Sessions and there will be more news soon."
Eminem will play the Glasgow Summer Sessions ahead of his headline slots at this year's Reading and Leeds festivals.
The US star is expected to release a new album later this year, after merchandise on his official store indicated that he will return to music in 2013.
Watch the music video for Eminem's 'I'm Not Afraid' below: