The Grammy-winning band are to kick off the first race of the NASCAR season with a special musical set on Sunday, February 24.
Clay Cook, one of the band's vocalists and guitar players, will also be singing the National Anthem just before the 55th annual Daytona 500 begins.
Fox is broadcasting the Daytona 500 live from the Daytona International Speedway in Florida.
Zac Brown Band have garnered more than 55 award nominations in the past 11 years, including a Grammy win for 'Best New Artist' in 2010.
Their latest studio album Uncaged topped the US album chart last summer.
Watch Zac Brown Band's 'The Wind' music video below: