The 'Suit & Tie' singer confirmed on his Twitter page that he will appear at the famous venue on Sunday, February 10, the same day as his Grammy Awards performance.
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Tickets for Timberlake's Hollywood Palladium show - in support of his forthcoming album The 20/20 Experience - go on sale tomorrow (Saturday, February 2) at 12pm PT (3pm ET). The show is billed to start at 9pm.
The musician and actor is due to make his live comeback tomorrow at a Super Bowl weekend benefit show in New Orleans.
[Justin Timberlake and Jay-Z film the video for 'Suit & Tie']
Timberlake will also perform at this month's Brit Awards, organisers confirmed on Friday afternoon (February 1).
The annual awards show takes place on Wednesday, February 20 at The O2 in London.
Watch the official lyric video for 'Suit & Tie' by Justin Timberlake and Jay-Z below: