The boyband have covered Blondie's classic hit 'One Way or Another' for this year's Comic Relief single, but the song leaked online earlier this week ahead of its official release.
"It is very disappointing that the song has been leaked and we are currently investigating the matter," a spokesperson for Syco told The Mirror.
Streams of the track have been removed from YouTube, but are still available to hear on alternative websites.
"I hear the charity single has been leaked. Very sad to see," Louis Tomlinson tweeted. "Don't forget together we are trying to raise money for comic relief."
Photo gallery - 25 years of Comic Relief:
One Direction will release their version of 'One Way or Another' on February 17 and will perform the track at the Brit Awards at London's O2 Arena on February 20.
Listen to a clip of One Direction's 'One Way or Another' below: