The singer will perform a rendition of 'Mirrors', which he unveiled following his set at the Grammy Awards on Sunday night (February 10).
The track is currently available as a free download when fans pre-order Timberlake's upcoming third studio album The 20/20 Experience.
The star also confirmed plans to embark on a world tour in support of his new record, saying the shows are "going to be a lot of fun".
One Direction, Taylor Swift, Robbie Williams, Emeli Sandé and Muse are among the other artists confirmed to perform at this year's Brit Awards.
The ceremony will take place at London's O2 Arena on February 20 and broadcast live on ITV.
Listen to Justin Timberlake's new song 'Mirrors' below: