The 'Interstate Love Song' band released a statement on Wednesday (February 27) confirming that the group will move in a new creative direction.
A group statement read: "Stone Temple Pilots have announced they have officially terminated Scott Weiland."
There were no further details provided on a replacement for Weiland or what led up to the personnel change.
Weiland's firing from Stone Temple Pilots was announced just one day after he spoke out to deny that there were any problems within the band.
"STP has not broken up," Weiland told Rolling Stone, adding: "I haven't quit. I haven't been fired."
Weiland fronted Stone Temple Pilots during their meteoric rise to alternative rock superstardom in the 1990s, when the band scored hits with 'Big Bang Baby', 'Plush' and 'Sour Girl'.
Stone Temple Pilots took an indefinite hiatus in 2003 following the promotion of their studio album Shangri-La Dee Da, only to reunite five years later.
Their self-titled sixth studio album earned a positive critical response when it was released in 2010.
Weiland is expected to launch a solo tour next week that will see him perform throughout North America in March.