The second track to be taken from Timberlake's album The 20/20 Experience climbed since the midweek chart update to stay at the top.
One Direction's Comic Relief single 'One Way or Another (Teenage Kicks)' rises back up four places to two.
Pink and Nate Ruess climb again to a new peak of three with 'Just Give Me a Reason'. Bruno Mars drops two to four with 'When I Was Your Man'. Bastille also fall two to five with 'Pompeii'.
Nicole Scherzinger enters at six with new single 'Boomerang', despite topping the chart in the midweek update.
Macklemore and Ryan Lewis slip two to seven with former number one 'Thrift Shop'. Bridgit Mendler falls one to eight with 'Ready or Not'.
Avicii and Nicky Romero are down one at nine with 'I Could Be the One'. Disclosure and AlunaGeorge re-enter the Top 10 at ten with 'White Noise'.
Nelly makes a return to the chart at 17 with new track 'Hey Porsche'. K Koke scores a top 20 debut with Rita Ora collaboration 'Lay Down Your Weapons' at 18.
Taylor Swift's new single '22' rises 40 places to 26 ahead of its official release next month.
Christina Perri climbs back 75 spots to 27 with 'A Thousand Years', following The Twilight Saga: Breaking Dawn - Part 2's release on DVD.
American Idol runner-up Blake Lewis jumps 133 places to 38 with 'Your Touch'.
Listen to Justin Timberlake's 'Mirrors' below:
Source: Official Charts Company
> This week's albums chart