The collection will be released on October 7 through Sony Music to celebrate the famous club's 20th anniversary.
G-A-Y The Album will include remixes of popular hits that are exclusive and not available elsewhere digitally.
Madonna's 'Hung Up (SDP EXTENDED MIX)' will open disc one, which also features songs by Adele, Cheryl Cole, Justin Timberlake and Nicki Minaj.
The Dirty Version of Icona Pop's recent global smash 'I Love It' heads up disc two, with further remixed versions of hits by Little Mix, Calvin Harris, JLS and Celine Dion.
Kylie Minogue, Cher, Spice Girls and M People are among the artists on disc three, which concludes with Dolly Parton's classic hit '9 To 5'.
Rihanna, Lady Gaga, Madonna, the Spice Girls and One Direction are just a handful of the artists who have performed at G-A-Y over the past 20 years.
"I can't believe that G-A-Y is 20 years old," said founder Jeremy Joseph. "It's been an incredible journey of highs and lows.
"I never had a vision for G-A-Y, just to create something I enjoyed and hoped others would as well.
"G-A-Y has always been about supporting artists at the beginning of their careers and supporting them as they grow, and in return as they get bigger, they continue to perform at G-A-Y."
The full tracklisting for G-A-Y The Album is as follows (*** denotes an exclusive song, otherwise unavailable digitally):
1. Madonna - 'Hung Up' SDP EXTENDED MIX***
2. Adele - 'Rolling In The Deep' (Villa Remix)
3. Katy Perry - 'Teenage Dream' (Manhattan Clique Edit)
4. Rihanna - 'Diamonds' (The Bimbo Jones Vocal Edit)
5. Lady Gaga - 'Poker Face' (Dave Audé Edit)
6. P!nk - 'So What' (Bimbo Jones Radio Mix)
7. Carly Rae Jepsen - 'Call Me Maybe' (Almighty Club Mix)
8. Jessie J - 'Domino' (Myon And Shane Remix)***
9. Cheryl - 'Call My Name' (Wideboys Remix Radio Edit)
10. The Pussycat Dolls - 'Don't Cha' (Ralphi Rosario Mix)
11. The Saturdays - 'All Fired Up' (The Alias Radio Edit)
12. Justin Timberlake feat. Timbaland - 'SexyBack' (Linus Loves Remix)***
13. Olly Murs - 'Troublemaker' (Cutmore Radio Edit)
14. One Direction - 'What Makes You Beautiful'
15. Nicki Minaj - 'Starships'
16. Britney Spears - 'Toxic' (Armand Van Helden Remix - Edit)
17. Girls Aloud - 'Girls Aloud Megamix'
1. Icona Pop feat Charli XCX - 'I Love It - Dirty Version'
2. Little Mix - 'Wings' (The Alias Radio Edit)
3. Calvin Harris feat. Florence Welch - 'Sweet Nothing' (Extended Mix)***
4. Rita Ora - 'How We Do (Party)' (Gustavo Scorpio Radio Mix - Dirty)***
5. Alexandra Burke - 'Bad Boys' (Moto Blanco Radio Mix)
6. Leona Lewis - 'Bleeding Love' (Moto Blanco Remix Radio Edit)***
7. McFly - 'Shine A Light'
8. JLS - 'The Club Is Alive' (Wideboys Stadium Mix)***
9. Kelly Clarkson - 'Stronger (What Doesn't Kill You)' (Project 46 Radio Edit)
10. Danni Minogue Vs. Flower Power - 'You Won't Forget About Me' (Vocal Radio Edit)***
11. Melanie C - 'I Turn To You' (Hex Hector Radio Mix)***
12. Sugababes - 'Push The Button' (DJ Prom International Edit)
13. Christina Aguilera - 'Beautiful' (Peter Rauhofer Radio Edit)***
14. Jennifer Lopez - 'Waiting For Tonight' (Hex's Momentous Radio Mix)***
15. Celine Dion - 'My Heart Will Go On' (Tony Moran Mix)***
16. Loreen - 'Euphoria'
17. Iggy Azalea - 'Bounce' (Danny Howard Remix)
18. Sam And The Womp - 'Bom Bom' (Radio Edit)
19. Ke$ha - 'We R Who We R' (Fred Falke Radio Mix)***
1. Kylie Minogue - 'Better The Devil You Know' (The Mad March Hare Mix)
2. Cher - 'Believe' (Almighty Definitive Remix)
3. Spice Girls - 'Spice Up Your Life' (Morales Radio Mix)
4. Whigfield - 'Saturday Night' (Radio Mix)
5. Steps - 'Last Thing On My Mind' (Wip't Up In The Disco Mix)***
6. Gina G - 'Just A Little Bit' (Motiv8 Radio Edit)
7. M People - 'One Night In Heaven'
8. Sophie Ellis-Bextor - 'Murder On The Dancefloor'
9. Kerry Ellis - 'Defying Gravity' (G-A-Y Remix)
10. Aqua - 'Turn Back Time' (Metro Scuba Club Mix)***
11. Alex Party - 'Don't Give Me Your Life'
12. Mark Ronson feat. Amy Winehouse - 'Valerie'
13. Shakira - 'Whenever, Wherever'
14. Five - 'Keep on Movin''
15. Wham! - 'Wake Me Up Before You Go-Go'
16. S Club 7 - 'S Club Party'
17. Take That Feat. Lulu - 'Relight My Fire'
18. Dolly Parton - '9 To 5'
Listen to Madonna's 'Hung Up' SDP Extended Mix below: