The singer has teamed up with R Kelly for new track 'Do What U Want', which has been lifted from her forthcoming album ARTPOP.
Gaga released the track digitally in the US earlier today (October 21) and has seen it rise to number one on the digital sales chart in a matter of hours.
Justin Bieber released 'Hold Tight' as part of his #MusicMondays campaign at the same time, with the song just behind Gaga at number two.
Meanwhile, Taylor Swift's new song 'Better Than Fiction' - which leads the soundtrack for new Paul Potts biopic One Chance - has shot up to number five since its release earlier this morning.
Elsewhere, Lorde holds firm at three with 'Royals', Katy Perry is currently at four with 'Roar' and Eminem's 'Rap God' is at six.
Listen to Lady Gaga's new song 'Do What U Want' below: