The electronic duo will also be joined on stage by a number of musicians who collaborated on their album Random Access Memories, according to Rolling Stone.
'Get Lucky' stars Pharrell Williams and Nile Rodgers will be among those involved, along with session players Chris Caswell, Nathan East, Omar Hakim and Paul Jackson Jr.
The ceremony marks Daft Punk's first live performance following the release of their most recent album, and will be their first televised show since their stint at the 2008 Grammys with Kanye West.
The pair are also nominated for four awards at the event - Album of the Year and Best Dance and Electronica Album for Random Access Memories, and Record of the Year and Best Pop Duo/Group Performance for 'Get Lucky'.
Pink, Kendrick Lamar and Imagine Dragons will also perform on the night.
The 2014 Grammy Awards will be broadcast live on CBS in the US on January 26 at 8pm ET.
Grammy Awards 2014: All the major nominees for 56th annual ceremony
Watch the video for Daft Punk's 'Instant Crush' below: