The country singer will make her Glastonbury debut in the 'golden oldie' slot on the Sunday afternoon, performing on the Pyramid Stage.
Speaking to Good Morning Britain about her preparations for the weekend, she laughed: "I grew up in a mud hole! I grew up on a farm, [there was] worse stuff than mud that we had to walk around in the barnyard - so we're used to all that!"
"I won't be in the mud, will I? I'll probably wear my galoshes up to the stage… but I'm going to sing in my high heels - mud or no mud!"
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Alongside her current live show, Parton is also working on a new stage show and movie about her life and career.
"It's the story of my life with a lot of original music, and of course we'll use some of the pieces people would expect, but I'm trying to do that as a stage musical and also probably a movie - the story of my life. So that would be good," she said.
"I've lived a long time, I've done a lot of stuff - I think there's enough to make a movie and a stage play!"
Glastonbury 2014 will take place this June 25-29 and will be headlined by Arcade Fire, Metallica and Kasabian.